Özgürün, Baturay10.07.20192019-07-1010.07.20192019-07-102018Özgürün, B. (2018). A simple and low cost structured illumination microscopy using a pico-projector. Conference on Single Molecule Spectroscopy and Superresolution Imaging XI. San Francisco, CA, January 27-28, 2018. https://dx.doi.org/10.1117/12.228679097815106148640277-786X1996-756Xhttps://dx.doi.org/10.1117/12.2286790https://hdl.handle.net/20.500.12511/3797Conference on Single Molecule Spectroscopy and Superresolution Imaging XI -- JAN 27-28, 2018 -- San Francisco, CAWOS: 000456502700004Here, development of a low-cost structured illumination microscopy (SIM) based on a pico-projector is presented. The pico-projector consists of independent red, green and blue LEDs that remove need for an external illumination source. Moreover, display element of the pico-projector serves as a pattern generating spatial light modulator. A simple lens group is employed to couple light from the projector to an epi-illumination port of a commercial microscope system. 2D sub SIM images are acquired and synthesized to surpass the diffraction limit using 40x (0.75 NA) objective. Resolution of the reconstructed SIM images is verified with a dye-and-object object and a fixed cell sample.eninfo:eu-repo/semantics/closedAccessSuper ResolutionMicroscopySIMPico-ProjectorStructured IlluminationConference Object1050010.1117/12.2286790N/AN/A