Ergan Şahin, AyçaKarasoy Yeşilada, AyşinYalçın, ÖzbenGüler, Eray MetinErbek, HarunKarabıyık, Damla2021-10-132021-10-132021Ergan Şahin, A., Karasoy Yeşilada, A., Yalçın, Ö., Güler, E. M., Erbek, H. ve Karabıyık, D. (2021). Hydrogen-rich saline reduces tissue injury and improves skin flap survival on a rat hindlimb degloving injury model. Journal of Plastic, Reconstructive and Aesthetic Surgery, 74(9), 2095-2103. https://dx.doi.org/10.1016/j.bjps.2020.12.0451748-68151878-0539https://dx.doi.org/10.1016/j.bjps.2020.12.045https://hdl.handle.net/20.500.12511/8440Background: Degloving injuries represent a challenge in plastic surgery. The aim of this study is to acknowledge the protective effects of hydrogen-rich saline (HRS) solution on a rat hindlimb degloved skin flap. Methods: Twenty-one Sprague–Dawley rats were divided into three groups (control, saline and HRS). Degloving injury model was established, and flaps were sutured back following 5 min of ischemia. The control group did not receive any treatment. The saline group received intraperitoneal physiological saline (10 ml/kg) and the HRS group received intraperitoneal HRS solution (10 ml/kg) postoperatively and daily for 5 days after the operation. Skin samples were obtained for histological, immunohistochemical and biochemical evaluations. Results: Inflammation was lower in the HRS compared with saline (p = 0.02) and control (p = 0.004) groups. Edema was lower in the HRS compared with saline (p = 0.02) and control (p = 0.001) groups. Malondialdehyde (MDA) level was lower in the HRS than the control group (p = 0.01). Total antioxidant level was higher in the HRS compared with saline (p = 0.009) and control (p = 0.03) groups. Total oxidant level was lower in the HRS than the control group (p = 0.02). Oxidative stress index was lower in the HRS compared with saline (p = 0.001) and control (p = 0.0001) groups`. Vascular proliferation was higher in the HRS compared with the control group (p = 0.01). Conclusion: Repeated HRS injections after trauma increased the viability of skin flap in rat degloving injury model by decreasing local tissue injury, due to its antioxidant, anti-inflammatory and angiogenic effects.eninfo:eu-repo/semantics/embargoedAccessDegloving InjuryFlapHydrogen-Rich SalineIschemia/Reperfusion InjuryArticle7492095210310.1016/j.bjps.2020.12.045Q2Q1