Cabıoğlu, NeslihanÇetin Aktaş, EsinEmiroğlu, SelmanTükenmez, MustafaÖzkurt, EnverMüslümanoğlu, Mahmutİğci, AbdullahÖzmen, VahitDeniz, GünnurDinççağ, Ahmet SaitGüven, Yusuf İzzettin2023-01-052023-01-052023Cabıoğlu, N., Çetin Aktaş, E., Emiroğlu, S., Tükenmez, M., Özkurt, E., Müslümanoğlu, M. ... Güven, Y. İ. (2023). Ozone therapy restores immune dysfunction in refractory idiopathic granulomatous mastitis as a novel potential therapeutic approach. Cell Biology International, 47(1), 228-237. https://dx.doi.org/10.1002/cbin.119531065-69951095-8355https://dx.doi.org/10.1002/cbin.11953https://hdl.handle.net/20.500.12511/10246Immunological dysfunction has been suggested to play a major role in the pathogenesis of idiopathic granulomatous mastitis (IGM). We recently showed that ozone therapy was effective in patients with steroid-resistant IGM. This study assessed alterations in intracellular cytokine expression patterns in different T-lymphocyte subsets after ozone therapy in refractory IGM. Peripheral blood T lymphocyte subsets (CD8(+), CD4(+), CD4(+)CD25(+) CD127(-)) were analyzed via flow-cytometry for intracellular cytokine expressions IFN-gamma, TNF-alpha, IL-10, and TGF-beta before and after completion of 4-month systemic ozone therapy. Ozone therapy significantly increased the CD4(+)IFN-gamma(+) (p = 0.032), CD4(+)TNF-alpha(+) (p = 0.028), and the CD8(+) TNF-alpha(+) (p =0.012) T cells. In contrast, significant decreases in CD4(+) IL-10(+) (p = 0.047) and CD8(+)IL-10(+) T cells (p = 0.022) and CD4(+)CD25(+) CD127(-//low) Treg cells secreting TGF-beta (p = 0.005) were found after ozone therapy. When patients were analyzed according to the response to ozone therapy, patients with a complete remission were more likely to have increased CD3(-)CD16(+)CD56(+) natural killer cells (p = 0.0027) and decreased CD19(+)B lymphocytes (p = 0.046) following ozone therapy. Our results suggest that ozone therapy stimulated a T-helper-1 response associated with IFN-gamma production and downregulation of TGF-beta expression in CD4(+)CD25(+)CD127(-) Treg cells. These alterations in the immune system following ozone therapy can improve wound healing and restore immune dysfunction in patients with refractory IGM.eninfo:eu-repo/semantics/embargoedAccessIdiopathic Granulomatous MastitisIFN??IL?10Ozone TherapyTGF??TNF??Article47122823710.1002/cbin.11953Q30008837780000012-s2.0-8514218544236378588Q2