Şakul, Ayşe ArzuOkur, Mehmet Evren2022-06-242022-06-242022Şakul, A. A. ve Okur, M. E. (2022). Beta-sitosterol and its antinociceptive mechanism action. Journal of Faculty of Pharmacy of Ankara University, 46(2), 238-252. http://doi.org/10.33483/jfpau.882831q1015-3918http://doi.org/10.33483/jfpau.882831qhttps://hdl.handle.net/20.500.12511/9540Objective: In this study, the possible central antinociceptive activity of beta-sitosterol is investigated along with its association of stimulation of opioidergic, serotonergic, adrenergic, and cholinergic receptors to mice central analgesia because of the beta-sitosterol administration. Material and Method: The beta-sitosterol was administrated to mice in various doses, such as 5, 10 and 20 mg/kg. Then, the mice analyzed via hot-plate and tail-flick assay to investigate the possible antinociceptive effects of beta-sitosterol. Additionally, in order to associate the mechanism of action mechanism, 20 mg/kg of beta-sitosterol was intraperitoneally administered to the animal which were previously pre-treated with opioid antagonist naloxone (5 mg/kg), serotonin 5-HT2A/2C receptor antagonist ketanserin (1 mg/kg), serotonin 5-HT3 receptor antagonist – ondansetron (1 mg/kg), ?2-adrenoceptor antagonist yohimbine (1 mg/kg) and muscarinic antagonist atropine (5 mg/kg), as well as nicotinic antagonist mecamylamine (1 mg/kg). Result and Discussion: The antinociceptive effect of beta-sitosterol was confirmed as dose-dependent for 5, 10, and 20 mg/kg doses in tail-flick and hot-plate tests. It can be concluded that beta-sitosterol promotes central antinociception effects associated with the spinal and supraspinal mediated cholinergic and opioidergic modulation.eninfo:eu-repo/semantics/closedAccessAntinociceptionBeta-SitosterolCholinergic ReceptorsOpioidergic ReceptorsArticle46223825210.33483/jfpau.882831q2-s2.0-85131801768Q3