Kaya, Yasin EmreKaraarslan, NumanYılmaz, İbrahimYaşar Şirin, DuyguYaman, OnurÖzbek, HanefiAteş, Özkan2021-02-052021-02-052020Kaya, Y. E., Karaarslan, N., Yılmaz, İ., Yaşar Şirin, D., Yaman, O., Özbek, H. ... Ateş, Ö. (2020). Investigation of the effects of tigecycline, a semi-synthetic derivative of minocycline, on chondrocyte cultures. Annals of Medical Research, 27(4), 1082-1087. https://doi.org/10.5455/annalsmedres.2019.11.7802636-7688https://doi.org/10.5455/annalsmedres.2019.11.780https://hdl.handle.net/20.500.12511/6533Abstract Aim: Although antibiotics are generally well-tolerated, they may have cytotoxic effects. The present randomized, double-blind, in vitro study aimed to investigate the effects of tigecycline on cartilage tissue cells and the extracellular matrix. Material and Methods: Cartilage tissues of patients (n = 8) were used for the preparation of primary cell cultures. Tigecycline-treated cell cultures served as the study group. Non-treated cell cultures served as the control group. Analyses were performed at 0, 24, 48, and 72 h in both groups. The results obtained were statistically evaluated. The alpha significance value was determined to be 0.05. Results: Proliferation remained unchanged in the tigecycline-treated cell cultures. The gene expressions of the markers involved in anabolic pathways increased in the tigecycline-treated cell cultures. The results obtained were statistically significant (P < 0.05). Conclusion: Although tigecycline had no toxic effect on the chondrocyte cell cultures and caused no damage to the extracellular matrix, the present study was performed in an in vitro environment.eninfo:eu-repo/semantics/openAccessChondroadherin GeneCartilage Oligomeric ProteinInterleukin-1betaMatrix MetalloproteinasePrimary Chondrocyte CultureTigecyclineArticle2741082108710.5455/annalsmedres.2019.11.780