Macit, ÇağlarEde Pazarbaşı, SerenYılmaz Karaoğlu, SümeyyeTunali Akbay, TuğbaKarakaya Çimen, Fatma BediaErcan, FerihaŞener, Göksel2023-10-032023-10-032023Macit, Ç., Ede Pazarbaşı, S., Yılmaz Karaoğlu, S., Tunali Akbay, T., Karakaya Çimen, F. B., Ercan, F. ... Şener, G. (2023). Panax ginseng extract ameliorates methotrexate-induced multi-organ damage via the regulation of oxidative stress. Journal of Research in Pharmacy, 27(5), 1974-1988. https://doi.org/10.29228/jrp.4772630-6344https://doi.org/10.29228/jrp.477https://hdl.handle.net/20.500.12511/11517Oxidative damage plays an important role in organ toxicities caused by methotrexate (MTX). This study aimed to determine the antioxidant effects of Panax ginseng (PxG) extract against MTX-induced liver, lung, ileum and kidney damage. Twenty-four Sprague Dawley male rats (weight 250-300 g) were used in the study. The animals were randomly separated into three groups: a) Control, b) MTX-treated (MTX) and c) MTX+PxG-treated (MTX+PxG) groups. MTX was administered intraperitoneally at 20 mg/kg, as a single dose to MTX and MTX+PxG groups. PxG was administered orally at 100 mg/kg to the MTX+PxG group for five days. Saline was given to the control and MTX groups for 5 days. At the end of the experiment, liver, lung, ileum, and kidney samples were obtained. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), glutathione-S-transferase (GST) and tissue factor (TF) activities were determined in all tissues. In addition, histological examinations were done through light microscopy. GraphPad Prism 5v. was used for statistics, and p[removed].eninfo:eu-repo/semantics/embargoedAccessAntioxidant ActivityIleumKidneyLiverLungMethotrexatePanax Ginseng ExtractArticle2751974198810.29228/jrp.477N/A0010691831000192-s2.0-85171323419Q3