Başer, Kemal Hüsnü CanKaradağ, Ayşe EsraBiltekin, Sevde NurErtürk, MuratDemirci, Fatih2023-02-132023-02-132023Başer, K. H. C., Karadağ, A. E. Biltekin, S. N., Ertürk, M. ve Demirci, F. (2023). In Vitro Antiviral Evaluations of Coldmix®: An Essential Oil Blend against SARS-CoV-2. Current Issues in Molecular Biology, 45(1), 677-684. https://dx.doi.org/10.3390/cimb450100451467-30371467-3045https://dx.doi.org/10.3390/cimb45010045https://hdl.handle.net/20.500.12511/10421Coldmix® is a commercially available Eucalyptus aetheroleum and, Abies aetheroleum blend for medicinal applications. In this present study, the in vitro antiviral potential of Coldmix®, and its major constituents 1,8-cineole and ?-pinene were evaluated by using the in vitro ACE2 enzyme inhibition assay as well as the direct contact test against SARS-CoV-2. The observed ACE2 enzyme inhibitory activity of Coldmix®, 1,8-cineole, and ?-pinene were 72%, 88%, and 80%, respectively; whereas in the direct contact test in the vapor phase, the destruction of the virus was 79.9% within 5 min and 93.2% in the 30th min, respectively. In a similar Coldmix® vapor phase setup using the in vitro cytotoxicity cell assay, E6 VERO healthy cells were experimentally not affected by toxicity. According to the promising initial antiviral results of Coldmix® and the individually tested constituents, detailed further in vivo evaluation using different virus classes is suggested.enAttribution 4.0 Internationalinfo:eu-repo/semantics/openAccessAbiesACE2AntiviralCoronavirusEssential OilsEucalyptusArticle45167768410.3390/cimb45010045Q30009145384000012-s2.0-8514704520636661531Q2