Mutlu, Yaşa GülSevcik, JoanKiss, Joseph ElmerLister, JohnMoore, Linda R.Donnenberg, Albert D.2024-03-112024-03-112024Mutlu, Y. G. Sevcik, J., Kiss, J. E., Lister, J., Moore, L. R. ve Donnenberg, A. D. (2024). Predicting the CD34 content of mobilized peripheral blood leukapheresis products: Single institution experience over 20 years. Cytotherapy, 26(2), 171-177. https://dx.doi.org/10.1016/j.jcyt.2023.09.0051465-32491477-2566https://dx.doi.org/10.1016/j.jcyt.2023.09.005https://hdl.handle.net/20.500.12511/12354Background aims: Since the standardization of CD34 measurement by flow cytometry, predictors of leukapheresis CD34 yield have played a pivotal role in planning donor leukaphereses. We describe here a single institution's experience with a multivariate predictor that was used for 2,929 products without alteration for 20 years. Methods: The ordinary least squares regression model variables included log peripheral CD34 count, collection duration (3- versus 4 -hours), collection number, donor sex, and transplant type. Results: During the study period we changed flow cytometers twice and leukapheresis instruments once. During the Cobe Spectra era the predictor explained 90% of the variability in CD34 collection yield for autologous transplants (r(2) = 0.90), and 70% for allogeneic transplants with an overall sensitivity to predict a CD34 yield of >= 1 x 10(6)/kg of 97.7%, and specificity of 81.4%. Conclusions: Implemented prospectively with real-time result reporting, the model allowed us to predict CD34 yield with both 3- and 4 -hour collection scenarios. Given this guidance, 3 -hour collections were selected by the clinical team 25% of the time, saving patient leukapheresis time and resources. When faced with a prediction of <1 x 10(6) CD34/kg, the clinical team chose to defer collection 72% of the time. In instances where leukapheresis was performed despite a poor predicted outcome, 85% of patients collected on the Cobe Spectra, and 92% of patients collected on the Optia, failed to collect at least 1 x 10(6) CD34/kg. A revised model is tested retrospectively on Optia data, and suggestions for further improvements are discussed.eninfo:eu-repo/semantics/embargoedAccessCD34Flow CytometryLeukapheresisMobilizationPredictorArticle26217117710.1016/j.jcyt.2023.09.005Q200117078730000137930293