Ceylan, Hasan HüseyinBilsel, KeremBüyükpınarbaşılı, NurCeylan, HamidErdil, MehmetTuncay, İbrahimŞen, Cengiz10.07.20192019-07-1010.07.20192019-07-102016Ceylan, H. H., Bilsel, K., Büyükpınarbaşılı, N., Ceylan, H., Erdil, M., Tuncay, İ. ... Şen, C. (2016). Can chondral healing be improved following microfracture? The effect of adipocyte tissue derived stem cell therapy. Knee, 23(3), 442-449. https://dx.doi.org/10.1016/j.knee.2015.11.0080968-01601873-5800https://dx.doi.org/10.1016/j.knee.2015.11.008https://hdl.handle.net/20.500.12511/3362WOS: 000378963600017PubMed ID: 27068292Background: We aimed to investigate the effect of adipose tissue-derived mesenchymal stem cells (ADSCs) on chondral healing using the microfracture (MF) technique. Methods: Thirty male rabbits were randomly divided into three groups. Standard cylindrical osteochondral defects (OCDs) were created in the weight-bearing areas of the medial condyles of all the right knees; the defects were four millimeters in diameter and two millimeters in depth. The control group (group A) was restricted to spontaneous healing. For group B, we performed MF with a 1.5-mm drill. For group C, we applied MF using the same method and then applied 3 x 10(6) ADSCs to the defect area. At eight weeks post-operation, the subjects were sacrificed, and the distal femoral joint surfaces were evaluated histopathologically for chondral healing. The samples were scored according to the International Cartilage Repair Society (ICRS) scale. Results: The results for group C were significantly better than those for group A in terms of the surface properties (p = 0.003). The matrix evaluation was better for group A than for group C (p = 0.01). The cell distribution, cell viability and subchondral bone parameters were similar between the groups (p = 0.198, p = 0.387 and p = 0.699). The cartilage mineralization parameter was better for group C than for group A (p = 0.001). The signs of healing were better for group C than for group B, but the differences were not significant (p = 0.185). Conclusions: Improvements with additional ADSC treatments were not statistically significant in cases in which ADSC treatment was compared with isolated MF treatment.eninfo:eu-repo/semantics/openAccessAdipocyte Tissue Derived Stem CellOsteochondral DefectMicrofractureChondral HealingCartilage RepairArticle23344244910.1016/j.knee.2015.11.008Q3Q1