Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract
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Erişim
info:eu-repo/semantics/embargoedAccessAttribution 4.0 United Stateshttp://creativecommons.org/licenses/by/4.0/us/Tarih
2019Üst veri
Tüm öğe kaydını gösterKünye
Okur, M. E., Karakaş, N., Karadağ, A. E., Uludağ, D. ve Çiçek Polat, D. (2019). Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. Istanbul Journal of Pharmacy, 49(3), 154-160. http://doi.org/10.26650/IstanbulJPharm.2019.19035Özet
Opuntia ficus-indica fruits are claimed to possess several health-beneficial properties. This study was carried out to determine the phytochemicals as well as to compare antioxidant activity and cell viability of the methanol extract of OFI-fruits. Antioxidant activity was evaluated by various in vitro tests such as DPPH and ABTS. LC-MS and HPLC analyses were then performed. The activity of the OFI-fruit extract on viability of U87-MG (glioblastoma multiform), HT-29 (colon cancer) and human primary fibroblast cells was investigated by determining viable cells on a luciferase based system (Cell TiterGlo; Promega; USA). The results showed that OFI-fruits were a source of antioxidant compounds. Moreover, the extract was found to have rich phenolic-flavonoid contents. Quercetin was found in the extract according to LC-MS and HPLC studies. A remarkable increased cell death in the HT-29 and U87 cell lines was detected in a concentration-dependent manner (1-10mg/mL). Among these cell lines, HT-29 responded to OFI-fruit treatment more sensitively to even the lowest dose (1mg/mL). The results of this study showed that the OFI-fruit has significant antioxidant capacity and leads to strong cytotoxic activity on various cancer cells. Therefore, it can be suggested that OFI-fruits may have chemo-preventive potential and can be studied further to clarify cytotoxic abilities.
Kaynak
Istanbul Journal of PharmacyCilt
49Sayı
3Koleksiyonlar
- Makale Koleksiyonu [295]
- Makale Koleksiyonu [276]
- Makale Koleksiyonu [3672]
- TR-Dizin İndeksli Yayınlar Koleksiyonu [2175]
- WoS İndeksli Yayınlar Koleksiyonu [6465]
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