dc.contributor.author | Yıldırım, Süleyman | |
dc.contributor.author | Thompson, Mitchell | |
dc.contributor.author | Jacobs, Anna | |
dc.contributor.author | Zurawski, Daniel | |
dc.contributor.author | Kirkup, Benjamin | |
dc.date.accessioned | 10.07.201910:49:13 | |
dc.date.accessioned | 2019-07-10T20:03:00Z | |
dc.date.available | 10.07.201910:49:13 | |
dc.date.available | 2019-07-10T20:03:00Z | |
dc.date.issued | 2016 | en_US |
dc.identifier.citation | Yıldırım, S., Thompson, M., Jacobs, A., Zurawski, D. ve Kirkup, B. (2016). Evaluation of parameters for high efficiency transformation of acinetobacter baumannii. Scientific Reports, 6. https://dx.doi.org/10.1038/srep22110 | en_US |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://dx.doi.org/10.1038/srep22110 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/3784 | |
dc.description | WOS: 000370802200001 | en_US |
dc.description | PubMed ID: 26911658 | en_US |
dc.description.abstract | Acinetobacter baumannii is an emerging, nosocomial pathogen that is poorly characterized due to a paucity of genetic tools and methods. While whole genome sequence data from several epidemic and environmental strains have recently become available, the functional characterization of genes is significantly lagging. Efficient transformation is one of the first steps to develop molecular tools that can be used to address these shortcomings. Here we report parameters allowing high efficiency transformation of A. baumannii. Using a multi-factorial experimental design we found that growth phase, voltage, and resistance all significantly contribute to transformation efficiency. The highest efficiency (4.3 x 10(8) Transformants/mu g DNA) was obtained at the stationary growth phase of the bacterium (OD 6.0) using 25 ng of plasmid DNA under 100 Ohms resistance and 1.7 kV/cm voltage. The optimized electroporation parameters reported here provide a useful tool for genetic manipulation of A. baumannii. | en_US |
dc.description.sponsorship | Military Infectious Diseases Research Program (MIDRP); Defense Medical Research and Development Program (DMRDP) | en_US |
dc.description.sponsorship | We are grateful to Professor Luis Actis for providing us with the plasmid pWH1266. This work was supported by multiple grants from the Military Infectious Diseases Research Program (MIDRP) and the Defense Medical Research and Development Program (DMRDP). The findings and opinions expressed herein belong to the authors and do not necessarily reflect the official views of the WRAIR, the US Army, or the Department of Defense. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Evaluation of parameters for high efficiency transformation of acinetobacter baumannii | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Scientific Reports | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-2752-1223 | en_US |
dc.identifier.volume | 6 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1038/srep22110 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.scopusquality | Q1 | en_US |