dc.contributor.author | Kara, Özlem | |
dc.contributor.author | Şeşeoğulları Dirihan, Roda | |
dc.contributor.author | Sayın Özel, Gülsüm | |
dc.contributor.author | Tezvergil Mutluay, Arzu | |
dc.contributor.author | Üşümez, Aslıhan | |
dc.date.accessioned | 2021-10-13T12:49:52Z | |
dc.date.available | 2021-10-13T12:49:52Z | |
dc.date.issued | 2021 | en_US |
dc.identifier.citation | Kara, Ö., Şeşeoğulları Dirihan, R., Sayın Özel, G., Tezvergil Mutluay, A. ve Üşümez, A. (2021). Inhibition of cathepsin-K and matrix metalloproteinase by photodynamic therapy. Dental Materials, 37(10), e485-e492. https://dx.doi.org/10.1016/j.dental.2021.08.015 | en_US |
dc.identifier.issn | 0109-5641 | |
dc.identifier.issn | 1879-0097 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.dental.2021.08.015 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/8443 | |
dc.description.abstract | Objectives: The objective of this study was to determine the effects of antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) and toluidine blue (TB) on protease activity (matrix-bound cathepsin K and matrix metalloproteinase (MMP) and dentin bond strength. Methods: Caries-free human third molars were assigned to five groups: 1—control group, 2—application of ICG with activation using an 810 nm diode (aPDT), 3—application of ICG, 4—application of TB with activation using a 660 nm diode (aPDT), and 5—application of TB. For the enzymatic investigation, dentin beams were incubated for either 3 days or 3 weeks. Aliquots of the incubation media were analyzed by ELISA for CTX (C-terminal cross-linked telopeptide of type I Collagen) and ICTP (cross-linked carboxy-terminal telopeptide of type I collagen). For microtensile bond strength testing (μTBS), composite resins were layered onto the tooth surface; the samples were then subjected to μTBS. Kruskall–Wallis and Mann–Whitney U tests were applied for statistical analysis of CTX and ICTP, one way-ANOVA and Tukey's test were applied for statistical analysis of μTBS. Results: Pretreating the dentin matrices with aPDT decreased the endogenous protease activity. ICG with laser activation resulted in the highest μTBS. Therefore, aPDT should be considered as a treatment method because it can reduce MMP-mediated dentin degradation and increase the μTBS. Significance: Inhibiting endogenous protease activity improves the stability of the dentin–adhesive bond and the durability of the bond strength. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Inc. | en_US |
dc.rights | info:eu-repo/semantics/embargoedAccess | en_US |
dc.subject | Antimicrobial Photodynamic Therapy | en_US |
dc.subject | CTX | en_US |
dc.subject | ICTP | en_US |
dc.subject | Indocyanine Green | en_US |
dc.subject | Microtensile Bond Strength | en_US |
dc.subject | Toluidine Blue | en_US |
dc.title | Inhibition of cathepsin-K and matrix metalloproteinase by photodynamic therapy | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Dental Materials | en_US |
dc.department | İstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Protetik Diş Tedavisi Ana Bilim Dalı | en_US |
dc.authorid | 0000-0001-8833-5259 | en_US |
dc.identifier.volume | 37 | en_US |
dc.identifier.issue | 10 | en_US |
dc.identifier.startpage | e485 | en_US |
dc.identifier.endpage | e492 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1016/j.dental.2021.08.015 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.scopusquality | Q1 | en_US |