dc.contributor.author | Olcay, Keziban | |
dc.contributor.author | Taşlı, Pakize Neslihan | |
dc.contributor.author | Pamukçu Güven, Esra | |
dc.contributor.author | Yalçın Ülker, Gül Merve | |
dc.contributor.author | Esen Öğüt, Emine | |
dc.contributor.author | Çiftçioǧlu, Elif | |
dc.contributor.author | Kıratlı, Binnur | |
dc.contributor.author | Şahin, Fikrettin | |
dc.date.accessioned | 2020-04-22T08:17:27Z | |
dc.date.available | 2020-04-22T08:17:27Z | |
dc.date.issued | 2020 | en_US |
dc.identifier.citation | Olcay, K., Taşlı, P. N., Pamukçu Güven, E., Yalçın Ülker, G. M., Esen Öğüt, E., Çiftçioǧlu, E. ... Şahin, F. (2020). Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements. Journal of Applied Oral Science, 28. https://dx.doi.org/10.1590/1678-7757-2019-0215 | en_US |
dc.identifier.issn | 1678-7757 | |
dc.identifier.issn | 1678-7765 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/5199 | |
dc.identifier.uri | https://dx.doi.org/10.1590/1678-7757-2019-0215 | |
dc.description.abstract | Objective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodology: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers' instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6-8 h. Statistical analyses included Kruskal-Wallis, Mann-Whitney U, and Friedman and Wilcoxon signed rank tests. Results: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p<0.05) with all tricalcium silicate-based materials at day 14. Tube formation by HUVECs showed a significant increase with all tested materials (p<0.05). Conclusion: The tricalcium silicate-based materials showed potential for angiogenic stimulation of all stem cell types and significantly enhanced tube formation by HUVECs. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Bauru School of Dentistry, University of Sao Paulo | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Angiogenesis Inducing Agents | en_US |
dc.subject | Dental Cements | en_US |
dc.subject | Regenerative | en_US |
dc.subject | Endodontics | en_US |
dc.subject | Stem Cells | en_US |
dc.title | Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Journal of Applied Oral Science | en_US |
dc.department | İstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Endodonti Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Ağız, Diş ve Çene Cerrahisi Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-2168-710X | en_US |
dc.authorid | 0000-0002-0150-6762 | en_US |
dc.identifier.volume | 28 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1590/1678-7757-2019-0215 | en_US |
dc.identifier.wosquality | Q2 | en_US |
dc.identifier.scopusquality | Q1 | en_US |