dc.contributor.author | Çakan, Orhan | |
dc.contributor.author | Hacıhasanoğlu Çakmak, Neziha | |
dc.contributor.author | Bayramoğlu, Feyza | |
dc.contributor.author | Yiğitbaşı, Türkan | |
dc.date.accessioned | 2023-03-22T12:21:00Z | |
dc.date.available | 2023-03-22T12:21:00Z | |
dc.date.issued | 2023 | en_US |
dc.identifier.citation | Çakan, O., Hacıhasanoğlu Çakmak, N., Bayramoğlu, F. ve Yiğitbaşı, T. (2023). The optimal method to measure polyamines in serum by using HPLC fluorescence detector. Acta Pharmaceutica Sciencia, 61(1), 91-106. https://dx.doi.org/10.23893/1307-2080.APS6107 | en_US |
dc.identifier.issn | 1307-2080 | |
dc.identifier.uri | https://dx.doi.org/10.23893/1307-2080.APS6107 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/10661 | |
dc.description.abstract | Measurement of polyamines (putrescine, spermidine and spermine) is important in the monitoring of many metabolic events, as well as in cancer prognosis. However, the lack of method standardization and validation for the measurement of poly-amines is a major obstacle. In this study, sample preparation, gradient program, chromatography column, sample concentration studies were attempted and an analysis method was developed for the detection of polyamines in serum by using HPLC fluorescence detector in order to reduce the difficulties experienced with the existing methodologies. This method was validated analytically in accordance with EMA and FDA’s guidelines. In this study, Waters Nova-Pak C18 A chromatography column with 3.9 mm, 150 mm and 4 µm specifications was used for the first time. Acetonitrile was used instead of methanol. Thanks to the newly developed gradient, peak purity and sufficient peak separation were ensured and accurate, sensitive, reliable and reproducible method was developed and validated. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Istanbul Medipol University | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Derivatization | en_US |
dc.subject | HPLC | en_US |
dc.subject | N-Acetyl-L Cysteine | en_US |
dc.subject | O-Phthalaldehyde | en_US |
dc.subject | Polyamines | en_US |
dc.title | The optimal method to measure polyamines in serum by using HPLC fluorescence detector | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Acta Pharmaceutica Sciencia | en_US |
dc.department | İstanbul Medipol Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-6183-5015 | en_US |
dc.authorid | 0000-0002-0757-9743 | en_US |
dc.authorid | 0000-0002-0675-1839 | en_US |
dc.identifier.volume | 61 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 91 | en_US |
dc.identifier.endpage | 106 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.23893/1307-2080.APS6107 | en_US |
dc.institutionauthor | Hacıhasanoğlu Çakmak, Neziha | |
dc.institutionauthor | Bayramoğlu, Feyza | |
dc.institutionauthor | Yiğitbaşı, Türkan | |
dc.identifier.scopus | 2-s2.0-85149203618 | en_US |
dc.identifier.scopusquality | Q3 | en_US |